Regulatory

Part:BBa_K2510026:Design

Designed by: Alma Chapet--Batlle   Group: iGEM17_Paris_Bettencourt   (2017-10-27)


O-P22C2 - T7 - O-P22C2 - O-HKcI promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The promoter was designed based on the work of Zang et al. using their library of insulated operators.

Source

This part was designed by the team and synthesized.

References

Zong, Y., Zhang, H., Lyu, C., Ji, X., Hou, J., Guo, X., Ouyang, Q. and Lou, C. (2017). Insulated transcriptional elements enable precise design of genetic circuits. Nature Communications, 8(1).